Background: Radiotherapy is one of the most prominent techniques used in cancer therapy. However, efforts for finding new effective radiosensitizers in improvement of radiotherapy efficacy have been continued yet. Wogonin is a natural flavonoid from Scutellaria litwinowii with known cytotoxic effects in some cancer cells.
Objective: To determine the potential of radiosensitizer effects of wogonin in a head and neck cancer cell line (HN5).
Methods: This study was carried out in Omid Hospital and Herbal Medicine Research Center (Mashhad, Iran). HN5 cells were cultured in DMEM medium and incubated with different concentrations of wogonin (25-500 µg/ml) and were exposed to (2-10 Gray [Gy]) γ-rays. Cell viability was quantified by MTT assay in wogonin cytotoxicity assay after 24, 48 and 72 hours and in coincident radiation and wogonin therapy after 72 hours. Data were analyzed by performing one-way ANOVA in SPSS (V.16). P-value ≤ 0.05 was considered significant.
Results: Wogonin decreased cell viability in HN5 cells as a time- and concentration-dependent manner. Wogonin could also enhance radiation-induced toxicity. For instance, while cell viability percentage in separate administration of wogonin (100 µM) and radiation (2 Gy) was significantly decreased to 53.98±2.76 and 74.97±1.05 respectively (p<0.001), wogonin 100 µM concurrent with 2 Gy radiation decreased the percentage of cell viability to 33.29±1.76 (p<0.001).
Conclusion: Concurrent use of wogonin and radiation increased radiation sensitivity and cell death in HN5 cells. Therefore, wogonin is a good radiosensitizer candidate for more study.
Keywords: Cytotoxicity; HN5 cell line; Radiosensitizing; Squamous cell of head and neck; Wogonin


» HTML Fulltext    » PDF Fulltext    » doi: 10.19082/7355

Current Issue

July-September 2019 (Volume 11, Issue 3)


Previous Issue

In the second issue of the journal Electronic Physician for 2019, we have several papers including four Randomized Controlled Trials, a model development study, a case report, an editorial, a letter to editor (LTE), and several original research including two studies with qualitative approach. Authors of this issue are from nine countries: Iran, The Netherlands, Sweden, Italy, India, Thailand, Saudi Arabia, United Arab Emirates (UAE), and Jordan. Read more...


The 6th World Conference on Research Integrity (WCRI) is to be held on June 2-5, 2019 in Hong Kong.

The WCRI is the largest and most significant international conference on research integrity. Since the first conference in Lisbon in 2007, it has given researchers, teachers, funding agencies, government officials, journal editors, senior administrators, and research students opportunities to share experiences and to discuss and promote integrity in research. Read more:


TDR Clinical Research and Development Fellowships

Call for applications

Deadline for submission: 7 March 2019, 16:00 (GMT)

TDR provides fellowships for early- to mid-career researchers and clinical trial staff (e.g. clinicians, pharmacists, medical statisticians, data managers, other health researchers) in low- and middle-income countries (LMICs) to learn how to conduct clinical trials. Read more:

Meta-Analysis Workshops in New York, USA, and London, UK, in April and May 2019

Don't miss this exceptional opportunity to learn how to perform and report a Meta-analysis correctly. Two Meta-analysis workshops are organized in April and May 2019 by Dr. Michael Borenstein in New York, USA (April 08-10, 2019) and London, UK (May 27-29).

About the Instructor

Dr. Michael Borenstein, one of the authors of Introduction to Meta-Analysis, is widely recognized for his ability to make statistical concepts accessible to researchers as well as to statisticians. He has lectured widely on meta-analysis, including at the NIH, CDC, and FDA. Read more: